Natural Occurrence and Enzymatic Synthesis of α-Nicotinamide Adenine Dinucleotide Phosphate
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منابع مشابه
Natural occurrence and enzymatic synthesis of alpha-nicotinamide adenine dinucleotide phosphate.
A pyridine nucleotide has been synthesized from cr-nicotinamide adenine dinucleotide and adenosine triphosphate with the use of a crude enzyme preparation from Azotobacfer uinelandii strain 0. Evidence is presented that this nucleotide is the (Y isomer of NADP, ar-NADP, which differs from &NADP in the configuration of the nicotinamide-ribosidic linkage. This evidence rests on a study of (a) spe...
متن کاملNatural Occurrence and Enzymatic Synthesis of a=Nicotinamide Adenine Dinucleotide Phosphate*
A pyridine nucleotide has been synthesized from cr-nicotinamide adenine dinucleotide and adenosine triphosphate with the use of a crude enzyme preparation from Azotobacfer uinelandii strain 0. Evidence is presented that this nucleotide is the (Y isomer of NADP, ar-NADP, which differs from &NADP in the configuration of the nicotinamide-ribosidic linkage. This evidence rests on a study of (a) spe...
متن کاملMeasurement of nicotinamide adenine dinucleotide & nicotinamide adenine dinucleotide phosphate in tomato leaves.
As part of an investigation of the growth of tomato plants supplied with either nitrate or ammonium nitrogen (6), leaves were assayed for NAD4 and NADP. Anderson and Vennesland (1) assayed NAD and NADP in various green tissues and reported recoveries between 50 % and 85 % of NAD and NADP added to spinach leaf extracts. Very low levels of NAD and NADP were recorded for tomato leaf extracts. Thes...
متن کاملInhibition of rat liver nicotinamide adenine dinucleotide kinase by reduced nicotinamide adenine dinucleotide phosphate.
Rat liver NAD kinase (ATP : NAD 2’-phosphotransferase, EC 2.7.1.23) was purified about 70-fold. The MichaelisMenten constants (Km) for NAD and ATP were 8 x 10e4 M and 2 x low3 M, respectively. NAD kinase activity was markedly inhibited by NADH and also NADPH. The Ki of NADH was approximately 1 X 10q4 M, and that of NADPH was approximately 5 X 10M5 M. Both inhibitions were competitive with NAD, ...
متن کاملNicotinamide Adenine Dinucleotide Phosphate - specific Isocitrate
Nicotiniamide adenine dintucleotide plhosphate-specific isocitrate dehydrogenase was extracted from etiolated pea (Pisurn satirum L.) seedlings and was purified 65-fold. The puirified enzyme exhibits one predoniinant protein band by pol acrylamide gel electrophoresis, w-hich corresponds to tiie dehy-drogenase activity as imieasuired by the nitro bluie tetrazolium technique. The reaction is read...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)95912-0